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Human Osteopontin protein, His tag (active)

Human Osteopontin protein, His tag (active). GTX00123-pro
Human Osteopontin protein, His tag (active). GTX00123-pro
Human Osteopontin protein, His tag (active). GTX00123-pro

Cat. No. GTX00123-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($209)
See all Osteopontin protein products

APPLICATION

Application Note

Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which would promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1×10⁵ cells/ml, and treated with or without OPN(100 ng/ml and 200 ng/ml) for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN.

Observed MW

60 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ile17~Asn287 (NP_000573.1)

Expression System

HEK293 cells

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

secreted phosphoprotein 1 , BNSP , BSPI , ETA-1 , OPN

Background

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Human Osteopontin protein, His tag (active). GTX00123-pro

GTX00123-pro Functional Assay Image

ELISA detection of secreted MCP-1 protein activation from MCF-7 cells induced by GTX00123-pro Human Osteopontin protein (active) for 24hrs.
Recombinant protein concentrations: 100, and 200 ng/ml.

Human Osteopontin protein, His tag (active). GTX00123-pro

GTX00123-pro Image

WB analysis of GTX00123-pro Human Osteopontin protein (active).

Human Osteopontin protein, His tag (active). GTX00123-pro

GTX00123-pro Image

SDS-PAGE analysis of GTX00123-pro Human Osteopontin protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 209