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Human Osteopontin protein, His tag (active)

Human Osteopontin protein, His tag (active). GTX01427-pro

Cat No. GTX01427-pro

Application

Functional Assay

Species

Human
Package
200 μg ($339),
100 μg ($259)

APPLICATION

Application Note

Measured by the ability of the immobilized protein to support the adhesion of HEK293 cells. When cells are added to coated plates (2 μg/ml, 100μl/well), approximately 60% will adhere after 1 hour of incubation at 37ºC.

Observed MW

60-65 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal poly His tagged; Met1-Asn314 of Human Osteopontin protein (NP_001035147.1)

Expression System

HEK293 cells

Purity

> 80% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BNSP,BSPI,ETA-1,OPN

Background

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Human Osteopontin protein, His tag (active). GTX01427-pro

GTX01427-pro Image

SDS-PAGE of 5 μg GTX01427-pro Human Osteopontin protein, His tag (active).

REFERENCE

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REVIEW

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