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Human PAPP A protein (active)

Human PAPP A protein (active). GTX00254-pro
Human PAPP A protein (active). GTX00254-pro
Human PAPP A protein (active). GTX00254-pro

Cat No. GTX00254-pro

Application Functional Assay
Species Human
Package
10 μg ($289)
APPLICATION

Application Note

Pregnancy-associated plasma protein A (PAPPA), also known as pappalysin-1, is a secreted protease whose main substrate is insulin-like growth factor binding proteins. PAPPA'Sproteolytic function is activated upon collagen binding. It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses (fetuses with an abnormal number of chromosomes). Besides, Plasminogen (Plg) has been identified as an interactor of PAPPA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PAPPA and recombinant human Plg. Briefly, PAPPA were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to Plg-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-PAPPA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of PAPPA and Plg was in a dose dependent manner.

Observed MW

33 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu317~Pro532 (NP_002572.2)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

pappalysin 1,ASBABP2,DIPLA1,IGFBP-4ase,PAPA,PAPP-A,PAPPA1

Background

This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES
Human PAPP A protein (active). GTX00254-pro

GTX00254-pro Functional Assay Image

Functional ELISA analysis of GTX00254-pro Human PAPP A protein (active) which can bind immobilized Plg protein.

Human PAPP A protein (active). GTX00254-pro

GTX00254-pro Image

SDS-PAGE analysis of GTX00254-pro Human PAPP A protein (active).

Human PAPP A protein (active). GTX00254-pro

GTX00254-pro Image

WB analysis of GTX00254-pro Human PAPP A protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289