APPLICATION
Application Note
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant Human PD1-His protein at 10 μg/ml (100 μl/well) can bind Human PD-L1 protein (active) (GTX01279-pro) with a linear range of 1.28-20 μg/ml.
2. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T cell lymphocytes. The ED₅₀ for this effect is typically 0.4-3 μg/ml.
Observed MW
51.7 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Batch dependent (please contact us for details)
Storage
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
C-terminal mouse IgG1-Fc tagged; Met1-Thr239 of Human PD-L1 protein (NP_054862.1)
Expression System
HEK293 cells
Purity
> 95% by SDS-PAGE.
Endotoxin
< 1.0 EU/μg (determined by LAL method)
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
B7-H,B7H1,PD-L1,PDCD1L1,PDCD1LG1,PDL1
Background
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
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GTX01279-pro Image
SDS-PAGE of 5 μg GTX01279-pro Human PD-L1 protein, mouse IgG1 Fc tag (active).
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GTX01279-pro Functional Assay Image
The protein binding activity was measured in a functional ELISA analysis of GTX01279-pro Human PD-L1 protein which can bind immobilized recombinant Human PD1-His protein.
Immobilized recombinant Human PD1-His protein : 10 μg/ml (100 μl/well)
GTX01279-pro : 1.28-20 μg/ml
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GTX01279-pro Functional Assay Image
The protein activity of GTX01279-pro Human PD-L1 protein was measured by its ability to inhibit anti-CD3 antibody (2 μg/ml) induced IL-2 secretion in human T lymphocytes.
ED₅₀ : 0.4-3 μg/ml
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REFERENCE
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REVIEW
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