The protein activity of GTX01279-pro Human PD-L1 protein was measured by its ability to inhibit anti-CD3 antibody (2 μg/ml) induced IL-2 secretion in human T lymphocytes.
ED₅₀ : 0.4-3 μg/ml

The protein binding activity was measured in a functional ELISA analysis of GTX01279-pro Human PD-L1 protein which can bind immobilized recombinant Human PD1-His protein.
Immobilized recombinant Human PD1-His protein : 10 μg/ml (100 μl/well)
GTX01279-pro : 1.28-20 μg/ml

SDS-PAGE of 5 μg GTX01279-pro Human PD-L1 protein, mouse IgG1 Fc tag (active).