Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids). Verified by N-terminal and Mass Spectrometry analyses (when applicable).
The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁶units/mg.
cross reactivity: Monkey, Mouse
Batch dependent (please contact us for details)
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.
Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
Beta chain: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT
≥ 98% by SDS-PAGE and HPLC.
< 1 EU/μg
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
platelet derived growth factor subunit A,platelet derived growth factor subunit B,PDGF-A,PDGF1,IBGC5,PDGF-2,PDGF2,SIS,SSV,c-sis