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Human PGD protein, His tag (active)

Human PGD protein, His tag (active). GTX67113-pro

Cat. No. GTX67113-pro

Application

 Functional Assay

Species

 Human
Package
20 μg ($219)
See all PGD protein products

APPLICATION

Application Note

Specific activity is > 10 units/mg, in which one unit oxidize 1.0 μmole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25ºC, in the presence of beta-NADP.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-HCl buffer (pH8.0), 1mM DTT, 100mM NaCl, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 503 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA

Expression System

E. coli

Purity

> 90% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphogluconate dehydrogenase , 6PGD

Background

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Human PGD protein, His tag (active). GTX67113-pro

GTX67113-pro Image

3 μg of GTX67113-pro Human PGD protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

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SDS
Glycerol.pdf
Package List Price ($)
$ 219