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Human PKC nu protein (active)

Cat No. GTX65399-pro

Application

Functional Assay

Species

Human
Package
5 μg ($379)

APPLICATION

Application Note

177 nmol phosphate incorporated into CREBTIDE substrate peptide per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% Glycerol.

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Expression System

Baculovirus (Sf9 cells)

Purity

≥ 80% by SDS-PAGE and HPLC.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase D3 , EPK2 , PKC-NU , PKD3 , PRKCN , nPKC-NU

Database

Research Area

REFERENCE

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REVIEW

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SDS
Glycerol.pdf