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Human PKR protein (active)

Human PKR protein (active). GTX00113-pro
Human PKR protein (active). GTX00113-pro
Human PKR protein (active). GTX00113-pro

Cat No. GTX00113-pro

Application

Functional Assay

Species

Human
Package
10 μg ($339)

APPLICATION

Application Note

Protein Kinase R (PKR) is activated by double-stranded RNA (dsRNA), the synthesis of which is caused virally. PKR can also be activated by the protein PACT or by heparin. It plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Besides, Cyclin Dependent Kinase 1 (CDK1) has been identified as an interactor of PKR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PKR and recombinant human CDK1. Briefly, PKR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CDK1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-PKR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of PKR and CDK1 was in a dose dependent manner.

Observed MW

32 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser224~Ile502 (NP_001129123.1)

Expression System

E. coli

Purity

> 80%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

eukaryotic translation initiation factor 2 alpha kinase 2 , EIF2AK1 , PKR , PPP1R83 , PRKR

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Human PKR protein (active). GTX00113-pro

GTX00113-pro Functional Assay Image

Functional ELISA analysis of GTX00113-pro Human PKR protein (active) which can bind immobilized CDK1 protein.

Human PKR protein (active). GTX00113-pro

GTX00113-pro Image

SDS-PAGE analysis of GTX00113-pro Human PKR protein (active).

Human PKR protein (active). GTX00113-pro

GTX00113-pro Image

WB analysis of GTX00113-pro Human PKR protein (active).

REFERENCE

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REVIEW

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