APPLICATION
Application Note
Specific activity is > 0.03 unit/mg. One unit will oxidize 1.0 μmole of L-proline to 1-pyrroline-5-carboxylate per minute in the presence of beta NAD at pH 10.0 at 25ºC.
PROPERTIES
Form
Liquid
Buffer
20mM Tris-HCl buffer (pH8.5), 10% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml
Region/Sequence
Full length protein, N-terminal His-Tag; Length: 339 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDLA TVSALRKMGV KLTPHNKETV QHSDVLFLAV KPHIIPFILD EIGADIEDRH IVVSCAAGVT ISSIEKKLSA FRPAPRVIRC MTNTPVVVRE GATVYATGTH AQVEDGRLME QLLSSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH ALHVLESGGF RSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL SPSGHTKLLP RSLAPAGKD
Expression System
E. coli
Purity
> 90% by SDS-PAGE.
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
pyrroline-5-carboxylate reductase 1 , ARCL2B , ARCL3B , P5C , P5CR , PIG45 , PP222 , PRO3 , PYCR
Background
This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Database
Research Area
DATA IMAGES
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GTX67150-pro Image
3 μg of GTX67150-pro Human PYCR1 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain
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REFERENCE
There are currently no references for Human PYCR1 protein, His tag (active) (GTX67150-pro). Be the first to share your publications with this product.
REVIEW
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