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Human Papilloma virus type 18 E7 antibody

Anti-Human Papilloma virus type 18 E7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133412
Anti-Human Papilloma virus type 18 E7 antibody used in Western Blot (WB). GTX133412

Cat No. GTX133412

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human papillomavirus type 18
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human papillomavirus

Immunogen

Full length Human Papilloma virus type 18 E7 recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886972

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Human papillomavirus Protein E7 , Human papillomavirus E7 , HPV virus Protein E7 , HPV virus E7

Research Area

DATA IMAGES

Anti-Human Papilloma virus type 18 E7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133412

GTX133412 ICC/IF Image

Human Papilloma virus type 18 E7 antibody detects Human Papilloma virus type 18 E7 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Human Papilloma virus type 18 E7 stained by Human Papilloma virus type 18 E7 antibody (GTX133412) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Human Papilloma virus type 18 E7 antibody used in Western Blot (WB). GTX133412

GTX133412 WB Image

Whole cell extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Human Papilloma virus type 18 E7 antibody (GTX133412) diluted at 1:1000.

REFERENCE

REVIEW

Human Papilloma virus type 18 E7 antibody Cat No. GTX133412

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 27-Jan-2022
Score :
Application Tested : WB
Sample Species : HPV18
Sample : HeLaS3 cells
Amount used : 25μg
Blocking : Can Get Signal/PVDF Blocking Reagent, 24°C, 1Hr
Primary Antibody : 1:2000, 24°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169