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Human Papillomavirus type 16 E7 antibody [HL1647]

Recombinant antibody Orthogonal
Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in Lateral Flow (Lateral Flow). GTX637228
Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in Western Blot (WB). GTX637228
Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in ELISA (ELISA). GTX637228
Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in ELISA (ELISA). GTX637228
Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in Western Blot (WB). GTX637228
Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637228

Cat. No. GTX637228

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1647

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

 Human papillomavirus type 16
Package
100 μl ($399),
25 μl ($169)
See all Human Papillomavirus type 16 E7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human papillomavirus type 16

Immunogen

Full length Human Papilloma virus type 16 E7

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in Lateral Flow (Lateral Flow). GTX637228

GTX637228 Lateral Flow Image

Detection of Human Papillomavirus type 16 E7 protein by lateral flow assay using the monoclonal antibody pair.

Capture: Human Papillomavirus type 16 E7 antibody (GTX637228 [HL1647])
Detection: Human Papillomavirus type 16 E7 antibody (GTX637546 [HL1821])

Samples (10 ng) :
1. HPV16 E7 protein (GTX133411-pro)
2. HPV18 E7 protein (GTX133412-pro)
3. Lysis buffer

Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in Western Blot (WB). GTX637228

GTX637228 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in ELISA (ELISA). GTX637228

GTX637228 ELISA Image

Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below.

Capture: Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (5 μg/mL)
Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (1 μg/mL)
Please notice that GTX637546 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637228. Please contact us for custom HRP-conjugated antibody.

Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in ELISA (ELISA). GTX637228

GTX637228 ELISA Image

Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below.

Capture: Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (5 μg/mL)
Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (1 μg/mL)
Please notice that GTX637228 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637546. Please contact us for custom HRP-conjugated antibody.

Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in Western Blot (WB). GTX637228

GTX637228 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Human Papilloma virus type 16 E7 antibody [HL1647] (GTX637228) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Human Papillomavirus type 16 E7 antibody [HL1647] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637228

GTX637228 ICC/IF Image

Human Papilloma virus type 16 E7 antibody [HL1647] detects Human Papilloma virus type 16 E7 protein by immunofluorescent analysis.
Sample: Caski cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Human Papilloma virus type 16 E7 stained by Human Papilloma virus type 16 E7 antibody [HL1647] (GTX637228) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations