Human Papillomavirus type 16 E7 antibody [HL1821]

Cat. No. GTX637546

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1821
Isotype	IgG
Application	WB, ELISA, Lateral Flow, Sandwich ELISA
Reactivity	Human papillomavirus type 16
Package	100 μl ($399), 25 μl ($169)

See all Human Papillomavirus type 16 E7 products

Datasheet

View product citations for antibody GTX637546 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ELISA	Assay dependent
Lateral Flow	Assay dependent
Sandwich ELISA	Assay dependent
Note : Lateral Flow Capture: GTX637228, Detection: GTX637546 Sandwich ELISA Capture : GTX637546 / GTX637228, Detection : GTX637228 / GTX637546. Please notice that GTX637228 / GTX637546 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637546 / GTX637228. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody was raised against Human Papillomavirus type 16 E7, and it does not cross-react with Human Papillomavirus type 18 E7.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human papillomavirus type 16

Immunogen

Full length Human Papillomavirus type 16 E7 recombinant protein

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

UniProt: P03129

Research Area

DATA IMAGES

GTX637546 Lateral Flow Image

Detection of Human Papillomavirus type 16 E7 protein by lateral flow assay using the monoclonal antibody pair.

Capture: Human Papillomavirus type 16 E7 antibody (GTX637228 [HL1647])
Detection: Human Papillomavirus type 16 E7 antibody (GTX637546 [HL1821])

Samples (10 ng) :
1. HPV16 E7 protein (GTX133411-pro)
2. HPV18 E7 protein (GTX133412-pro)
3. Lysis buffer

GTX637546 ELISA Image

Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below.

Capture: Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (5 μg/mL)
Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (1 μg/mL)
Please notice that GTX637546 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637228. Please contact us for custom HRP-conjugated antibody.

GTX637546 ELISA Image

Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below.

Capture: Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (5 μg/mL)
Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (1 μg/mL)
Please notice that GTX637228 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637546. Please contact us for custom HRP-conjugated antibody.

GTX637546 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637546 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

GTX637546 WB Image

Human papillomavirus protein (100 ng) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546). Be the first to share your experience with this product.

Submit a Review

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Human Papillomavirus type 16 E7 antibody [HL1821]

Cat. No. GTX637546

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Database

Research Area

DATA IMAGES

GTX637546 Lateral Flow Image

GTX637546 ELISA Image

GTX637546 ELISA Image

GTX637546 WB Image

GTX637546 WB Image

GTX637546 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

© 1998-2022 GeneTex, Inc. All rights reserved.

All products are for research use only—Not for use in diagnostic or therapeutic applications.