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Human Papillomavirus type 16 E7 antibody [HL1821]

Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in Western Blot (WB). GTX637546
Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in Lateral Flow (Lateral Flow). GTX637546
Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in ELISA (ELISA). GTX637546
Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in ELISA (ELISA). GTX637546
Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in Western Blot (WB). GTX637546
Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in Western Blot (WB). GTX637546

Cat. No. GTX637546

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1821

Isotype

IgG

Application

WB, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

Human papillomavirus type 16
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Lateral Flow
Capture: GTX637228, Detection: GTX637546

Sandwich ELISA
Capture : GTX637546 / GTX637228, Detection : GTX637228 / GTX637546. Please notice that GTX637228 / GTX637546 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637546 / GTX637228. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody was raised against Human Papillomavirus type 16 E7, and it does not cross-react with Human Papillomavirus type 18 E7.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human papillomavirus type 16

Immunogen

Full length Human Papillomavirus type 16 E7 recombinant protein

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in Western Blot (WB). GTX637546

GTX637546 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in Lateral Flow (Lateral Flow). GTX637546

GTX637546 Lateral Flow Image

Detection of Human Papillomavirus type 16 E7 protein by lateral flow assay using the monoclonal antibody pair.

Capture: Human Papillomavirus type 16 E7 antibody (GTX637228 [HL1647])
Detection: Human Papillomavirus type 16 E7 antibody (GTX637546 [HL1821])

Samples (10 ng) :
1. HPV16 E7 protein (GTX133411-pro)
2. HPV18 E7 protein (GTX133412-pro)
3. Lysis buffer

Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in ELISA (ELISA). GTX637546

GTX637546 ELISA Image

Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below.

Capture: Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (5 μg/mL)
Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (1 μg/mL)
Please notice that GTX637546 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637228. Please contact us for custom HRP-conjugated antibody.

Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in ELISA (ELISA). GTX637546

GTX637546 ELISA Image

Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below.

Capture: Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (5 μg/mL)
Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (1 μg/mL)
Please notice that GTX637228 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637546. Please contact us for custom HRP-conjugated antibody.

Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in Western Blot (WB). GTX637546

GTX637546 WB Image

Human papillomavirus protein (100 ng) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Human Papillomavirus type 16 E7 antibody [HL1821] used in Western Blot (WB). GTX637546

GTX637546 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations