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Human Papillomavirus type 16 E7 antibody

Anti-Human Papillomavirus type 16 E7 antibody used in Western Blot (WB). GTX133411

Cat. No. GTX133411

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human papillomavirus type 16
Package
100 μl ($399),
25 μl ($169)
See all Human Papillomavirus type 16 E7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human papillomavirus

Immunogen

Full length Human Papilloma virus type 16 E7 recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886971

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Human papillomavirus Protein E7 , Human papillomavirus E7 , HPV virus Protein E7 , HPV virus E7

Research Area

DATA IMAGES

Anti-Human Papillomavirus type 16 E7 antibody used in Western Blot (WB). GTX133411

GTX133411 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody (GTX133411) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zacharias NM et al. Cancers (Basel) 2024; 16 (5) Transcriptomic, Proteomic, and Genomic Mutational Fraction Differences Based on HPV Status Observed in Patient-Derived Xenograft Models of Penile Squamous Cell Carcinoma.
J?nsson J et al. Nucleic Acids Res 2024; 52 (1) : 316-336 A novel HPV16 splicing enhancer critical for viral oncogene expression and cell immortalization.
Fan J et al. Cell Genom 2023; 3 (1) : 100211 Multi-omics characterization of silent and productive HPV integration in cervical cancer.
Application : WB
Park S et al. Cancers (Basel) 2021; 13 (5) HIV-1 Protease Inhibitors Slow HPV16-Driven Cell Proliferation through Targeted Depletion of Viral E6 and E7 Oncoproteins.
Application : WB
Lo Cigno I et al. J Virol 2020; 94 (4) Human Papillomavirus E7 Oncoprotein Subverts Host Innate Immunity via SUV39H1-Mediated Epigenetic Silencing of Immune Sensor Genes.
Application : WB
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REVIEW

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Application Reference
Zacharias NM et al. Cancers (Basel) 2024; 16 (5) Transcriptomic, Proteomic, and Genomic Mutational Fraction Differences Based on HPV Status Observed in Patient-Derived Xenograft Models of Penile Squamous Cell Carcinoma.
J?nsson J et al. Nucleic Acids Res 2024; 52 (1):316-336 A novel HPV16 splicing enhancer critical for viral oncogene expression and cell immortalization.
Fan J et al. Cell Genom 2023; 3 (1):100211 Multi-omics characterization of silent and productive HPV integration in cervical cancer.
Application : WB
Park S et al. Cancers (Basel) 2021; 13 (5) HIV-1 Protease Inhibitors Slow HPV16-Driven Cell Proliferation through Targeted Depletion of Viral E6 and E7 Oncoproteins.
Application : WB
Zheng Y et al. J Virol 2020; 94 (20) Heterogeneous Nuclear Ribonucleoprotein A1 (hnRNP A1) and hnRNP A2 Inhibit Splicing to Human Papillomavirus 16 Splice Site SA409 through a UAG-Containing Sequence in the E7 Coding Region.
Application : WB
Lo Cigno I et al. J Virol 2020; 94 (4) Human Papillomavirus E7 Oncoprotein Subverts Host Innate Immunity via SUV39H1-Mediated Epigenetic Silencing of Immune Sensor Genes.
Application : WB
Carper MB et al. Cell Rep 2019; 29 (6):1660-1674.e7 An Immunocompetent Mouse Model of HPV16(+) Head and Neck Squamous Cell Carcinoma.
Application : WB
Zhao J et al. Nat Commun 2019; 10 (1):2300 Transforming activity of an oncoprotein-encoding circular RNA from human papillomavirus.
Application : WB
Jiawei Zhao et al. bioRxiv 2019; (Epub) Translation and Transforming Activity of a Circular RNA from Human Papillomavirus.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169