GeneTex
  • Country / Location Selection

United States (US)

Human Papillomavirus type 18 E7 antibody

Anti-Human Papillomavirus type 18 E7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133412
Anti-Human Papillomavirus type 18 E7 antibody used in Western Blot (WB). GTX133412

Cat. No. GTX133412

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human papillomavirus type 18
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human papillomavirus

Immunogen

Full length Human Papilloma virus type 18 E7 recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886972

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Human papillomavirus Protein E7 , Human papillomavirus E7 , HPV virus Protein E7 , HPV virus E7

Research Area

DATA IMAGES

Anti-Human Papillomavirus type 18 E7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133412

GTX133412 ICC/IF Image

Human Papilloma virus type 18 E7 antibody detects Human Papilloma virus type 18 E7 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Human Papilloma virus type 18 E7 stained by Human Papilloma virus type 18 E7 antibody (GTX133412) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Human Papillomavirus type 18 E7 antibody used in Western Blot (WB). GTX133412

GTX133412 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 18 E7 antibody (GTX133412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Human Papillomavirus type 18 E7 antibody Cat. No. GTX133412

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 27-Jan-2022
Score :
Application Tested : WB
Sample Species : HPV18
Sample : HeLaS3 cells
Amount used : 25μg
Blocking : Can Get Signal/PVDF Blocking Reagent, 24°C, 1Hr
Primary Antibody : 1:2000, 24°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169