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Human Plasminogen protein (active)

Human Plasminogen protein (active). GTX00216-pro
Human Plasminogen protein (active). GTX00216-pro
Human Plasminogen protein (active). GTX00216-pro

Cat No. GTX00216-pro

Application

Functional Assay

Species

Human
Package
10 μg ($209)

APPLICATION

Application Note

Plasmin is an important enzyme present in blood that degrades many blood plasma proteins, including fibrin clots. It is released as a zymogen called plasminogen (PLG) from the liver into the systemic circulation. Plasmin is a serine protease that acts to dissolve fibrin blood clots. Apart from fibrinolysis, plasmin proteolyses proteins in various other systems: It activates collagenases, some mediators of the complement system and weakens the wall of the Graafian follicle (leading to ovulation). It cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Plasmin, like trypsin, belongs to the family of serine proteases. Besides, Actin Beta (ACTb) has been identified as an interactor of PLG, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PLG and recombinant human ACTb. Briefly, PLG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-PLG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of PLG and ACTb was in a dose dependent manner.

Observed MW

38 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; confidential (NP_000292.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

plasminogen

Background

The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES

Human Plasminogen protein (active). GTX00216-pro

GTX00216-pro Functional Assay Image

Functional ELISA analysis of GTX00216-pro Human Plasminogen protein (active) which can bind immobilized ACTb protein.

Human Plasminogen protein (active). GTX00216-pro

GTX00216-pro Image

SDS-PAGE analysis of GTX00216-pro Human Plasminogen protein (active).

Human Plasminogen protein (active). GTX00216-pro

GTX00216-pro Image

WB analysis of GTX00216-pro Human Plasminogen protein (active).

REFERENCE

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REVIEW

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