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Human Plasminogen protein (active)

Human Plasminogen protein (active). GTX00419-pro
Human Plasminogen protein (active). GTX00419-pro
Human Plasminogen protein (active). GTX00419-pro

Cat No. GTX00419-pro

Application Functional Assay
Species Human
Package
10 μg ($209)
APPLICATION

Application Note

Plasminogen (PLG) as a zymogen releases plasmin from the liver into the systemic circulation. Plasmin is a serine protease that acts to dissolve fibrin blood clots. Apart from fibrinolysis, plasmin proteolyses proteins in various other systems: It activates collagenases, some mediators of the complement system and weakens the wall of the Graafian follicle (leading to ovulation). It cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Plasmin, like trypsin, belongs to the family of serine proteases. Besides, Insulin Like Growth Factor Binding Protein 3 (IGFBP3) has been identified as an interactor of PLG, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PLG and recombinant human IGFBP3. Briefly, PLG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IGFBP3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-PLG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of PLG and IGFBP3 was in a dose dependent manner.

Observed MW

35 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Reconstitute with 20 mM Tris (pH8.0), 150 mM NaCl to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu100~Cys352 (NP_000292.1)

Expression System

Yeast

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

plasminogen

Background

The protein encoded by this gene is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasmin dissolves fibrin in blood clots and is an important protease in many other cellular processes while angiostatin inhibits angiogenesis. Defects in this gene are likely a cause of thrombophilia and ligneous conjunctivitis. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2009]

Database

Research Area

DATA IMAGES
Human Plasminogen protein (active). GTX00419-pro

GTX00419-pro Functional Assay Image

Functional ELISA analysis of GTX00419-pro Human Plasminogen protein (active) which can bind immobilized IGFBP3 protein.

Human Plasminogen protein (active). GTX00419-pro

GTX00419-pro Image

SDS-PAGE analysis of GTX00419-pro Human Plasminogen protein (active).

Human Plasminogen protein (active). GTX00419-pro

GTX00419-pro Image

WB analysis of GTX00419-pro Human Plasminogen protein (active).

REFERENCE
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