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Human Progranulins protein (active)

Human Progranulins protein (active). GTX65641-pro
Human Progranulins protein (active). GTX65641-pro

Cat No. GTX65641-pro

Application

Functional Assay

Species

Human
Package
10 μg ($289)

APPLICATION

Application Note

Activates phospho-ERK1/2 in neuronal mouse P19 cells. Regulates food intake and body weight.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 0.2μm-filtered PBS. Reconstitute with 100 μl ddH₂O.

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.

Region/Sequence

Signal peptide and human progranulin (a.a. 1-593); reflects the native with no additional amino acid.

Expression System

HEK293 cells

Purity

≥ 98% by SDS-PAGE.

Endotoxin

< 0.01 EU/μg

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

granulin precursor , CLN11 , GEP , GP88 , PCDGF , PEPI , PGRN

Database

Research Area

DATA IMAGES

Human Progranulins protein (active). GTX65641-pro

GTX65641-pro Image

The effects on phospho-ERK1/2 and ERK1/2 by GTX65641-pro Progranulin protein in neuronal differentiated mouse P19 cells. Undifferentiated mouse P19 cells were induced to differentiated in 1μM retinoic acid (RA) in α-minimum essential medium (αMEM) containing 10% heat-treated fetal bovine serum on bacterial grade plates for 3~4 days to allow aggregates to form (generation of embryonic bodies). The aggregates were then plated on tissue culture grade plates in the absence of RA for 3~4days. To examine the induction of signal of phospho-ERK1/2 and ERK1/2, reactions were carried out at 37ºC over 0, 5, 10, 30, 60, 120mins, respectively by adding the recombinant protein (500ng/ml) to the neuronal differentiated mouse P19 cells, which were maintained with serum starvation for 24hrs. Treatment with Progranulin protein (GTX65641-pro) was performed in lanes 1, 2, 3, 4, 5, and 6 over 0, 5, 10, 30, 60, 120mins, respectively. GAPDH was used as loading control for western blotting.

Human Progranulins protein (active). GTX65641-pro

GTX65641-pro Image

Deglycosylation of GTX65641-pro Progranulin (human) protein. To examine the deglycosylation of human Progranulin, 1 μg of human progranulin is denatured with 1X glycoprotein denaturing buffer at 100ºC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37ºC. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (human) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 289