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Human RANK protein, His tag

Human RANK protein, His tag. GTX00262-pro
Human RANK protein, His tag. GTX00262-pro
Human RANK protein, His tag. GTX00262-pro

Cat. No. GTX00262-pro

Application

Functional Assay

Species

Human
Package
10 μg ($289)

APPLICATION

Application Note

RANK tumor necrosis factor receptor superfamily member 11A protein (TNFRSF11A) also known as receptor Activator of Nuclear Factor κ B (RANK) or TRANCE Receptoris a member of the tumor necrosis factor receptor (TNFR) molecular sub-family. TNFRSF11A is the receptor for RANK-Ligand (RANKL) and part of the RANK/RANKL/OPG signaling pathway that regulates osteoclast differentiation and activation. It is associated with bone remodeling and repair, immune cell function, lymph node development, thermal regulation, and mammary gland development. Besides, TNF Receptor Associated Factor 5 (TRAF5) has been identified as an interactor of TNFRSF11A, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF11A and recombinant human TRAF5. Briefly, TNFRSF11A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TRAF5-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TNFRSF11A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF11A and TRAF5 was in a dose dependent manner.

Observed MW

44 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Val330~Lys615 (NP_001257878.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF receptor superfamily member 11a , CD265 , FEO , LOH18CR1 , ODFR , OFE , OPTB7 , OSTS , PDB2 , RANK , TRANCER

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2012]

Database

  • Gene ID: 8792 TNFRSF11A
  • UniProt: Q9Y6Q6 TNFRSF11A

Research Area

DATA IMAGES

Human RANK protein, His tag. GTX00262-pro

GTX00262-pro Functional Assay Image

Functional ELISA analysis of GTX00262-pro Human RANK protein which can bind immobilized TRAF5 protein.

Human RANK protein, His tag. GTX00262-pro

GTX00262-pro Image

WB analysis of GTX00262-pro Human RANK protein.

Human RANK protein, His tag. GTX00262-pro

GTX00262-pro Image

SDS-PAGE analysis of GTX00262-pro Human RANK protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 289