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Human RPP40 protein, His tag

Human RPP40 protein, His tag. GTX00241-pro
Human RPP40 protein, His tag. GTX00241-pro
Human RPP40 protein, His tag. GTX00241-pro

Cat. No. GTX00241-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($299)
See all RPP40 protein products

APPLICATION

Application Note

Ribonuclease P (RNASEP) is a type of ribonuclease which cleaves RNA. RNase P is unique from other RNases in that it is a ribozyme – a ribonucleic acid that acts as a catalyst in the same way that a protein based enzyme would. Its function is to cleave off an extra, or precursor, sequence of RNA on tRNA molecules. Besides, Methyl CpG Binding Protein 2 (MECP2) has been identified as an interactor of RNASEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human RNASEP and recombinant human MECP2. Briefly, RNASEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MECP2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-RNASEP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of RNASEP and MECP2 was in a dose dependent manner.

Observed MW

28 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Cys49~Asn258 (NP_001273061.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribonuclease P/MRP subunit p40 , RNASEP1 , bA428J1.3

Database

DATA IMAGES

Human RPP40 protein, His tag. GTX00241-pro

GTX00241-pro Functional Assay Image

Functional ELISA analysis of GTX00241-pro Human RPP40 protein which can bind immobilized MECP2 protein.

Human RPP40 protein, His tag. GTX00241-pro

GTX00241-pro Image

SDS-PAGE analysis of GTX00241-pro Human RPP40 protein.

Human RPP40 protein, His tag. GTX00241-pro

GTX00241-pro Image

WB analysis of GTX00241-pro Human RPP40 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 299