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Human RSPO1 protein (active)

Cat No. GTX53852-pro

Application

Functional Assay

Species

Human
Package
20 μg ($199)
PRODUCT

Summary

Recombinant human R-Spondin-1 is a 26.7 kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS PAGE analysis under reducing conditions. Verified by N-terminal and Mass Spectrometry analyses (when applicable).

APPLICATION

Application Note

Assay #1: R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED₅₀ is 1.0-3.0 μg/ml.
Assay #2: Measured by its ability to induce Topflash reporter activity in HEK293 TCF/LEF Luc reporter cells. The ED₅₀ for this effect is typically 0.6-1.0 μg/ml in the presence of 50 ng/ml recombinant human BMP-2.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.

Region/Sequence

SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA

Expression System

CHO cells

Purity

≥ 95% by SDS-PAGE and HPLC.

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

R-spondin 1 , CRISTIN3 , RSPO

Database

Research Area

REFERENCE

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REVIEW

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