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Human S100A8 protein (active)

Human S100A8 protein (active). GTX00252-pro
Human S100A8 protein (active). GTX00252-pro
Human S100A8 protein (active). GTX00252-pro

Cat No. GTX00252-pro

Application Functional Assay
Species Human
Package
10 μg ($239)
APPLICATION

Application Note

S100 calcium-binding protein A8 (S100A8) also known as calgranulin A, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Besides, S100 Calcium Binding Protein A9 (S100A9) has been identified as an interactor of S100A8, thus a binding ELISA assay was conducted to detect the interaction of recombinant human S100A8 and recombinant human S100A9. Briefly, S100A8 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to S100A9-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-S100A8 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of S100A8 and S100A9 was in a dose dependent manner.

Observed MW

12 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Met1~Glu93 (NP_001306126.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

S100 calcium binding protein A8 , 60B8AG , CAGA , CFAG , CGLA , CP-10 , L1Ag , MA387 , MIF , MRP8 , NIF , P8

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES
Human S100A8 protein (active). GTX00252-pro

GTX00252-pro Functional Assay Image

Functional ELISA analysis of GTX00252-pro Human S100A8 protein (active) which can bind immobilized S100A9 protein.

Human S100A8 protein (active). GTX00252-pro

GTX00252-pro Image

SDS-PAGE analysis of GTX00252-pro Human S100A8 protein (active).

Human S100A8 protein (active). GTX00252-pro

GTX00252-pro Image

WB analysis of GTX00252-pro Human S100A8 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 239