APPLICATION
Application Note
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is less or equal to 10 ng/ml.
PROPERTIES
Form
Liquid
Buffer
PBS (pH7.4)
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
Length: 165 a.a. Sequence: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC
VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
Expression System
E. coli
Purity
> 95% by SDS-PAGE.
Endotoxin
< 1.0 EU/μg (determined by LAL method)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
KIT ligand , DCUA , DFNA69 , FPH2 , FPHH , KL-1 , Kitl , MGF , SCF , SF , SHEP7 , SLF
Background
This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX67152-pro Image
3 μg of GTX67152-pro Human SCF protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain
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REFERENCE
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REVIEW
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