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Human SDF1 protein (active)

Human SDF1 protein (active). GTX00099-pro
Human SDF1 protein (active). GTX00099-pro
Human SDF1 protein (active). GTX00099-pro

Cat No. GTX00099-pro

Application Functional Assay
Species Human
Package
10 μg ($269)
APPLICATION

Application Note

SDF1 (stromal cell-derived factor 1), also known as C-X-C motif chemokine 12, is a chemokine protein that has chemotaxis active on T-lymphocytes and monocytes. It is thought that SDF1 stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3; thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of SDF1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 μl cell suspension, 1x10⁶cells/ml in RPMI-1640 with 0. 5% FBS) and SDF1 (10 ng/ml, 30 ng/ml and 60 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). The migrated THP-1 cells in low chamber at low magnification (×100) randomly, and the migrated cells were counted at high magnification (×400). SDF1 is able to induce migration of THP-1 cells, and the optimum chemotaxis of SDF1 occurs at 10 ng/ml.

Observed MW

40 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Pro23~Lys89 (NP_000600.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-X-C motif chemokine ligand 12,IRH,PBSF,SCYB12,SDF1,TLSF,TPAR1

Background

This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Database

Research Area

DATA IMAGES
Human SDF1 protein (active). GTX00099-pro

GTX00099-pro Functional Assay Image

The chemotactic effect of GTX00099-pro Human SDF1 protein (active).
Upper: (A) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without SDF1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 with 10 ng/ml SDF1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

Human SDF1 protein (active). GTX00099-pro

GTX00099-pro Image

SDS-PAGE analysis of GTX00099-pro Human SDF1 protein (active).

Human SDF1 protein (active). GTX00099-pro

GTX00099-pro Image

WB analysis of GTX00099-pro Human SDF1 protein (active).

REFERENCE
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REVIEW
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