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Human SEMA3F protein (active)

Human SEMA3F protein (active). GTX00267-pro
Human SEMA3F protein (active). GTX00267-pro
Human SEMA3F protein (active). GTX00267-pro

Cat No. GTX00267-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

Semaphorin-3F (SEMA3F) is a member of semaphorins family. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Semaphorins not only guide axons in development, but also have major roles in immune function (classes 4, 6, and 7) and the development of bones. Class 3 semaphorins are one of the most versatile semaphorin classes, in which Sema3a is the most studied. Besides, SEMA3F is deleted or downregulated in many metastatic tumors. To test the effect of SEMA3F on inhibit cell proliferation,  human lung carcinoma cells A549 were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM  prior to the addition of various concentrations of recombinant human SEMA3F. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of A549 cells after incubation with SEMA3F for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 assay after incubation with recombinant SEMA3F for 48h. And SEMA3F significantly increased cell viability of A549 cells.

Observed MW

16 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Val350~Leu483 (NP_001305729.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

semaphorin 3F,SEMA-IV,SEMA4,SEMAK

Background

This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin loop and a C-terminal basic domain. This gene is expressed by the endothelial cells where it was found to act in an autocrine fashion to induce apoptosis, inhibit cell proliferation and survival, and function as an anti-tumorigenic agent. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES
Human SEMA3F protein (active). GTX00267-pro

GTX00267-pro Functional Assay Image

Cell suppression effect of GTX00267-pro Human SEMA3F protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated A549 cells cultured in DMEM for 48hrs.
(B) A549 cells cultured in DMEM, stimulated with 100 ng/ml SEMA3F for 48hrs.

Human SEMA3F protein (active). GTX00267-pro

GTX00267-pro Image

WB analysis of GTX00267-pro Human SEMA3F protein (active).

Human SEMA3F protein (active). GTX00267-pro

GTX00267-pro Image

SDS-PAGE analysis of GTX00267-pro Human SEMA3F protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 259