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Human SOD3 protein (active)

Human SOD3 protein (active). GTX00425-pro
Human SOD3 protein (active). GTX00425-pro

Cat No. GTX00425-pro

Application Functional Assay
Species Human
Package
10 μg ($249)
APPLICATION

Application Note

Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. According to the report, in a weakly alkaline buffer solution (pH 8.2) with N-tris (hydroxymethyl) amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measure the activity of recombinant human SOD3. The reaction was performed in adding 30 μl pyrogallol (50mmol/l) to 900 μl Tris-HCl (50mmol/l) in 1.5ml cuvette (1.0cm light path), rapidly mixing at 25ºC, then read at 325nm (zero the spectrophotometer using 50mmol/l Tris-HCl), record the OD value every 30 second for 6 times. Control the pyrogallol autoxidation rate at 0.70 OD/min. After, adding various concentrations of recombinant of SOD3 to 900 μl Tris-HCl (50mmol/l), incubated for 20min at 25ºC, then adding 30 μl pyrogallol (50mmol/l) to each tube, rapidly mixing and read at 325nm, record the OD value every 30 second for 6 times. Under these conditions, the enzyme amount of 50% per minute inhibition of pyrogallol autooxidation is defined as a unit. The specific activity of recombinant human SOD3 is 121.4 U/mg.

Observed MW

33/30 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Reconstitute with 20 mM Tris (pH8.0), 150 mM NaCl to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Trp19~Ala240 (NP_003093.2)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

superoxide dismutase 3,EC-SOD

Background

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the conversion of superoxide radicals into hydrogen peroxide and oxygen, which may protect the brain, lungs, and other tissues from oxidative stress. Proteolytic processing of the encoded protein results in the formation of two distinct homotetramers that differ in their ability to interact with the extracellular matrix (ECM). Homotetramers consisting of the intact protein, or type C subunit, exhibit high affinity for heparin and are anchored to the ECM. Homotetramers consisting of a proteolytically cleaved form of the protein, or type A subunit, exhibit low affinity for heparin and do not interact with the ECM. A mutation in this gene may be associated with increased heart disease risk. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES
Human SOD3 protein (active). GTX00425-pro

GTX00425-pro Image

SDS-PAGE analysis of GTX00425-pro Human SOD3 protein (active).

Human SOD3 protein (active). GTX00425-pro

GTX00425-pro Image

WB analysis of GTX00425-pro Human SOD3 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 249