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Human STIP1 protein, His tag (active)

Human STIP1 protein, His tag (active). GTX00445-pro

Cat. No. GTX00445-pro

Application

 Functional Assay

Species

 Human
Package
50 μg ($439)
See all STIP1 protein products

APPLICATION

Application Note

4 μM HOP Protein, when added to 2 μM AHA1 Protein (Aha1)-activated HSP90 (2 μM; His-tagged HSP90 beta) in 33 mM HEPES (pH 7.2) with 30 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 1.5 mM ATP in a 100 μl reaction at 37 ºC, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic HSP90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).

Observed MW

63 kDa.

PROPERTIES

Form

Liquid

Buffer

20mM HEPES buffer (pH7.2), 80mM NaCl, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full-length protein, N-terminal His-Tag; NP_006810.1

Expression System

E. coli

Purification

Affinity Purified

Purity

> 90%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

stress induced phosphoprotein 1 , HEL-S-94n , HOP , IEF-SSP-3521 , P60 , STI1 , STI1L

Background

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009]

Database

Research Area

DATA IMAGES

Human STIP1 protein, His tag (active). GTX00445-pro

GTX00445-pro Image

SDS-PAGE analysis of GTX00445-pro Human STIP1 protein (active).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 439