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Human Semaphorin 3A protein (active)

Human Semaphorin 3A protein (active). GTX00178-pro
Human Semaphorin 3A protein (active). GTX00178-pro
Human Semaphorin 3A protein (active). GTX00178-pro

Cat No. GTX00178-pro

Application Functional Assay
Species Human
Package
10 μg ($309)
APPLICATION

Application Note

The Semaphorin 3A (SEMA3A) which belongs to the semaphorin family can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Semaphorin 3A is secreted protein containing a Sema domain, an immunoglobulin C2-like domain and a basic domain near the carboxyl tail. It can be secreted by neurons and surrounding tissue to guide migrating cells and axons in the developing nervous system. Besides, Neuropilin 1 (NRP1) has been identified as an interactor of SEMA3A, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SEMA3A and recombinant human NRP1. Briefly, SEMA3A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NRP1-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-SEMA3A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of SEMA3A and NRP1 was in a dose dependent manner.

Observed MW

16 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Arg31~Cys141 (NP_006071.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

semaphorin 3A,COLL1,HH16,Hsema-I,Hsema-III,SEMA1,SEMAD,SEMAIII,SEMAL,SemD,coll-1

Background

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human Semaphorin 3A protein (active). GTX00178-pro

GTX00178-pro Functional Assay Image

Functional ELISA analysis of GTX00178-pro Human Semaphorin 3A protein (active) which can bind immobilized NRP1 protein.

Human Semaphorin 3A protein (active). GTX00178-pro

GTX00178-pro Image

SDS-PAGE analysis of GTX00178-pro Human Semaphorin 3A protein (active).

Human Semaphorin 3A protein (active). GTX00178-pro

GTX00178-pro Image

WB analysis of GTX00178-pro Human Semaphorin 3A protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 309