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Human Shh protein, His tag (active)

Human Shh protein, His tag (active). GTX01284-pro
Human Shh protein, His tag (active). GTX01284-pro

Cat No. GTX01284-pro

Application

Functional Assay

Species

Human
Package
100 μg ($409),
20 μg ($199)

APPLICATION

Application Note

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 cells. The ED₅₀ for this effect is typically 2-10 μg/ml.

Observed MW

24 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal poly His tagged; Met1-Gly197 of Human Shh protein (Q15465)

Expression System

HEK293 cells

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HHG1,HLP3,HPE3,MCOPCB5,SMMCI,ShhNC,TPT,TPTPS

Background

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human Shh protein, His tag (active). GTX01284-pro

GTX01284-pro Image

SDS-PAGE of 5 μg GTX01284-pro Human Shh protein, His tag (active).

Human Shh protein, His tag (active). GTX01284-pro

GTX01284-pro Functional Assay Image

The protein activity of GTX01284-pro Human Shh protein was mearured by its ability to induce alkaline phosphatase production by C3H10T1/2 cells.
ED₅₀ : 2-10 μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 409
$ 199