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Human Sorbitol Dehydrogenase protein (active)

Human Sorbitol Dehydrogenase protein (active). GTX67160-pro

Cat No. GTX67160-pro

Application

Functional Assay

Species

Human
Package
10 μg ($199)

APPLICATION

Application Note

Specific activity is > 15 units/mg, and is defined as the amount of enzyme that catalyze the reduction 1.0 μmole of D-fructose to D-sorbitol per minute at pH 7.5 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 8.5) with 10% glycerol, 1 mM DTT

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

Full length protein, Length: 357 a.a. Sequence: MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP

Expression System

E.coli

Purity

> 90% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sorbitol dehydrogenase , HEL-S-95n , SORD1

Background

Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]

Database

Research Area

DATA IMAGES

Human Sorbitol Dehydrogenase protein (active). GTX67160-pro

GTX67160-pro Image

3 μg of GTX67160-pro Human Sorbitol Dehydrogenase protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 199