GeneTex
  • Country / Location Selection

United States (US)

Human Syk protein (active)

Human Syk protein (active). GTX00233-pro
Human Syk protein (active). GTX00233-pro
Human Syk protein (active). GTX00233-pro

Cat No. GTX00233-pro

Application Functional Assay
Species Human
Package
10 μg ($319)
APPLICATION

Application Note

Spleen Tyrosine Kinase (SYK) is a member of the SYK family of tyrosine kinases. These non-receptor cytoplasmic tyrosine kinases share a characteristic dual SH2 domain separated by a linker domain. Within B and T cells respectively, SYK and Zap-70 transmit signals from the B-Cell receptor and T-Cell receptor. SYK plays a similar role in transmitting signals from a variety of cell surface receptors including CD74, Fc Receptor, and integrins. Besides, Sialic Acid Binding Ig Like Lectin 1 (SIGLEC2) has been identified as an interactor of SYK, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SYK and recombinant human SIGLEC2. Briefly, SYK were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SIGLEC2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-SYK pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of SYK and SIGLEC2 was in a dose dependent manner.

Observed MW

34 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asp374~Asn635 (NP_001128524.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

spleen associated tyrosine kinase,p72-Syk

Background

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES
Human Syk protein (active). GTX00233-pro

GTX00233-pro Functional Assay Image

Functional ELISA analysis of GTX00233-pro Human Syk protein (active) which can bind immobilized SIGLEC2 protein.

Human Syk protein (active). GTX00233-pro

GTX00233-pro Image

WB analysis of GTX00233-pro Human Syk protein (active).

Human Syk protein (active). GTX00233-pro

GTX00233-pro Image

SDS-PAGE analysis of GTX00233-pro Human Syk protein (active).

REFERENCE
There are currently no references for Human Syk protein (active) (GTX00233-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human Syk protein (active) (GTX00233-pro). Be the first to share your experience with this product.