APPLICATION
Application Note
Tissue Factor Pathway Inhibitor 2 (TFPI2) takes part in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. TFPI2 does not have any influence on thrombin. TFPI2 also can inhibit MMP activity, which can hydrolyze gelatin under certain conditions. Thus, the activity of TFPI2 can be measured by inhibit MMP-2 hydrolyze gelatin. Gelatin zymography is mainly used for the detection of the gelatinases, 2μg/ml was denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 8% gels) containing gelatin (1 mg/ml) with nonreducing conditions. After renaturation, incubate with various concentrations of recombinant human TFPI2, then staining with coomassie brilliant blue G250, active MMP-2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel; if the activity of MMP-2 inhibit by TFPI2, there was none white binds on the gel. MMP-2 can be inhibited by recombinant human TFPI2 at least 5μg/ml.
Observed MW
27 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 100 mM NaHCO₃ and 500 mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex. Lyophilized from 100 mM NaHCO₃ (pH 8.3) with 500 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Asp23~Phe235 (NP_001257932.1)
Expression System
E. coli
Purity
> 95%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tissue factor pathway inhibitor 2 , PP5 , REF1 , TFPI-2
Background
This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Database
Research Area
DATA IMAGES
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GTX00278-pro Functional Assay Image
Hydrolysis assay on gelatin (1 mg/ml) with nonreducing conditions induced by various concentrations of GTX00278-pro Human TFPI2 protein (active). MMP-2 protein can be inhibited by Human TFPI2 protein (active) at least 5 μg/ml.
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GTX00278-pro Functional Assay Image
MMP-2 inhibition assay by various concentrations of GTX00278-pro Human TFPI2 protein (active) on gelatin hydrolysis. MMP-2 can be inhibited by Human TFPI2 protein (active) at least 5 μg/mlL.
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GTX00278-pro Image
WB analysis of GTX00278-pro Human TFPI2 protein (active).
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GTX00278-pro Image
SDS-PAGE analysis of GTX00278-pro Human TFPI2 protein (active).
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REFERENCE
There are currently no references for Human TFPI2 protein, His tag (active) (GTX00278-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human TFPI2 protein, His tag (active) (GTX00278-pro). Be the first to share your experience with this product.
