GeneTex
  • Country / Location Selection

United States (US)

Human TIMP4 protein (active)

Human TIMP4 protein (active). GTX00229-pro
Human TIMP4 protein (active). GTX00229-pro
Human TIMP4 protein (active). GTX00229-pro

Cat No. GTX00229-pro

Application Functional Assay
Species Human
Package
10 μg ($299)
APPLICATION

Application Note

Tissue Inhibitors Of Metalloproteinase 4 (TIMP4) is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. Besides, Matrix Metalloproteinase 2 (MMP2) has been identified as an interactor of TIMP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TIMP4 and recombinant human MMP2. Briefly, TIMP4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to MMP2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TIMP4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TIMP4 and MMP2 was in a dose dependent manner.

Observed MW

24 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Cys32~Pro224 (NP_003247.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TIMP metallopeptidase inhibitor 4,TIMP-4

Background

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human TIMP4 protein (active). GTX00229-pro

GTX00229-pro Functional Assay Image

Functional ELISA analysis of GTX00229-pro Human TIMP4 protein (active) which can bind immobilized MMP2 protein.

Human TIMP4 protein (active). GTX00229-pro

GTX00229-pro Image

WB analysis of GTX00229-pro Human TIMP4 protein (active).

Human TIMP4 protein (active). GTX00229-pro

GTX00229-pro Image

SDS-PAGE analysis of GTX00229-pro Human TIMP4 protein (active).

REFERENCE
There are currently no references for Human TIMP4 protein (active) (GTX00229-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human TIMP4 protein (active) (GTX00229-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 299