Human TNF Alpha protein (active)

Cat No. GTX65267-pro

Application	Functional Assay
Species	Human
Package	50 μg ($299)

See all TNF alpha protein products

Datasheet

PRODUCT

Summary

Human TNF-alpha is a 17.4 kDa protein containing 157 amino acid residues.

APPLICATION

Application Note

The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 10⁷ units/mg.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 50 mM Tris (pH 8.5), 150 mM NaCl. Reconstitute with ddH₂O to 1.0 mg/ml.

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.

Expression System

E. coli

Purity

≥ 98% by SDS-PAGE and HPLC.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F

Database

Gene ID: 7124 TNF

Research Area

DATA IMAGES

GTX65267-pro Functional Assay Image

ED₅₀ < 0.05 ng/ml as determined by the cytolysis of murine L929 cells treated with actinomycin using commercial cell Proliferation colorimetric assay kit.

GTX65267-pro Image

Recombinant Protein analyzed using SDS-PAGE and stained with coomassie blue. Lanes are as follows: M - MW markers, 1, 3 and 5 μg purified TNF-α. The protein shows a predicted MW of ~ 17.4 kDa.