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Human TNF Alpha protein (active)

Human TNF Alpha protein (active). GTX65267-pro
Human TNF Alpha protein (active). GTX65267-pro
Human TNF Alpha protein (active). GTX65267-pro

Cat No. GTX65267-pro

Application

 Functional Assay

Species

 Human
Package
50 μg ($299)
See all TNF alpha protein products
PRODUCT

Summary

Human TNF-alpha is a 17.4 kDa protein containing 157 amino acid residues.

APPLICATION

Application Note

The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 10⁷ units/mg.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 50 mM Tris (pH 8.5), 150 mM NaCl. Reconstitute with ddH₂O to 1.0 mg/ml.

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.

Expression System

E. coli

Purity

≥ 98% by SDS-PAGE and HPLC.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F

Database

Research Area

DATA IMAGES

Human TNF Alpha protein (active). GTX65267-pro

GTX65267-pro Functional Assay Image

ED₅₀ < 0.05 ng/ml as determined by the cytolysis of murine L929 cells treated with actinomycin using commercial cell Proliferation colorimetric assay kit.

Human TNF Alpha protein (active). GTX65267-pro

GTX65267-pro Image

Recombinant Protein analyzed using SDS-PAGE and stained with coomassie blue. Lanes are as follows: M - MW markers, 1, 3 and 5 μg purified TNF-α. The protein shows a predicted MW of ~ 17.4 kDa.

Human TNF Alpha protein (active). GTX65267-pro

GTX65267-pro Image

SEC analysis of TNF-α on a Superose 6 Increase 5/150 column at 0.1mL/min in in 50 mM Na-phosphate, 300mM NaCl pH 7.2.

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Package List Price ($)
$ 299