Summary
Tumor Necrosis Factor-a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids (157 a.a.of the mature Human TNF-alpha and an N-terminal methionine) and having a molecular mass of 17.5 kDa.
Form
Lyophilized powder
Buffer
Reconstitute with ddH₂O but not less than 100 μg/ml. Lyophilized from 20mM PBS (pH7.2), 100mM NaCl.
Preservative
No preservative
Storage
Store at -20ºC or below. After reconstitution, keep as concentrated solution. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding a carrier protein (0.1% HSA or BSA), then aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Region/Sequence
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL.
Expression System
E. coli
Purity
> 95% by SDS-PAGE and HPLC.
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.