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Human TNF Receptor I protein (active)

Human TNF Receptor I protein (active). GTX00234-pro
Human TNF Receptor I protein (active). GTX00234-pro
Human TNF Receptor I protein (active). GTX00234-pro

Cat No. GTX00234-pro

Application Functional Assay
Species Human
Package
10 μg ($279)
APPLICATION

Application Note

Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFα). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. TNFRSF1B can inhibit cell apoptosis which induced by TNFα. Briefly, A549 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1μg/ml TNFα and various concentrations of recombinant human TNFRSF1B. After incubated for 96h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate at 37ºC for 1-4 hours. Apoptosis of A549 cells had been inhibit after incubation with TNFRSF1B for 96h observed by inverted microscope.

Observed MW

50 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Thr60~Tyr236 (NP_001056.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TNF receptor superfamily member 1A,CD120a,FPF,TBP1,TNF-R,TNF-R-I,TNF-R55,TNFAR,TNFR1,TNFR55,TNFR60,p55,p55-R,p60

Background

This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients. [provided by RefSeq, Sep 2016]

Database

Research Area

DATA IMAGES
Human TNF Receptor I protein (active). GTX00234-pro

GTX00234-pro Functional Assay Image

The suppression effect of GTX00234-pro Human TNF Receptor I protein (active) on TNF-alpha induced apoptosis. Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) A549 cells cultured in DMEM only contain 1 μg/ml TNFα for 96hrs.
(B) A549 cells cultured in DMEM contain 1 μg/ml TNF alpha and 0.1 ng/ml TNFRSF1B for 96hrs.

Human TNF Receptor I protein (active). GTX00234-pro

GTX00234-pro Image

SDS-PAGE analysis of GTX00234-pro Human TNF Receptor I protein (active).

Human TNF Receptor I protein (active). GTX00234-pro

GTX00234-pro Image

WB analysis of GTX00234-pro Human TNF Receptor I protein (active).

REFERENCE
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