APPLICATION
Application Note
TNF-a (Tumor necrosis factor), is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It has been reported that TNFa can inhibit the proliferation and induce apoptosis of A549 cells, besides, the concentration of IL-1β and IL-8 in cell supernatant will increase after stimulation. Therefore, a stimulation assay of TNFa was conducted using A549 cells. Briefly, A549 cells were incubated in DMEM with different concentrations of TNFa (1 ng/ml, 10 ng/ml, 100 ng/ml, 1000 ng/ml) for 8h, after which the concentration of IL-1β in the cell supernatant were detected by ELISA. IL-1β levels in the cell supernatant of A549 cells increased significantly after stimulation.
Observed MW
21 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Val77~Leu233 (NP_000585.2)
Expression System
E. coli
Purity
> 95%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F
Background
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX00102-pro Functional Assay Image
ELISA detection of secreted IL-1 beta activation from A549 cells induced by GTX00102-pro Human TNF alpha protein (active) for 8hrs.
Recombinant protein concentrations: 1, 10, 100, and 1000 ng/ml.
|
|
|
GTX00102-pro Functional Assay Image
ELISA detection of secreted IL-8 protein activation from A549 cells induced by GTX00102-pro Human TNF alpha protein (active) for 8hrs.
Recombinant protein concentrations: 1, 10, 100, and 1000 ng/ml.
|
|
|
GTX00102-pro Image
SDS-PAGE analysis of GTX00102-pro Human TNF alpha protein (active).
|
|
|
GTX00102-pro Image
WB analysis of GTX00102-pro Human TNF alpha protein (active).
|
REFERENCE
There are currently no references for Human TNF alpha protein, His tag (active) (GTX00102-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human TNF alpha protein, His tag (active) (GTX00102-pro). Be the first to share your experience with this product.
