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Human TNF alpha protein (active)

Human TNF alpha protein (active). GTX00102-pro
Human TNF alpha protein (active). GTX00102-pro
Human TNF alpha protein (active). GTX00102-pro
Human TNF alpha protein (active). GTX00102-pro

Cat No. GTX00102-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

TNF-a (Tumor necrosis factor), is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It has been reported that TNFa can inhibit the proliferation and induce apoptosis of A549 cells, besides, the concentration of IL-1β and IL-8 in cell supernatant will increase after stimulation. Therefore, a stimulation assay of TNFa was conducted using A549 cells. Briefly, A549 cells were incubated in DMEM with different concentrations of TNFa (1 ng/ml, 10 ng/ml, 100 ng/ml, 1000 ng/ml) for 8h, after which the concentration of IL-1β in the cell supernatant were detected by ELISA. IL-1β levels in the cell supernatant of A549 cells increased significantly after stimulation.

Observed MW

21 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Val77~Leu233 (NP_000585.2)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tumor necrosis factor,DIF,TNF-alpha,TNFA,TNFSF2,TNLG1F

Background

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human TNF alpha protein (active). GTX00102-pro

GTX00102-pro Functional Assay Image

ELISA detection of secreted IL-1 beta activation from A549 cells induced by GTX00102-pro Human TNF alpha protein (active) for 8hrs.
Recombinant protein concentrations: 1, 10, 100, and 1000 ng/ml.

Human TNF alpha protein (active). GTX00102-pro

GTX00102-pro Functional Assay Image

ELISA detection of secreted IL-8 protein activation from A549 cells induced by GTX00102-pro Human TNF alpha protein (active) for 8hrs.
Recombinant protein concentrations: 1, 10, 100, and 1000 ng/ml.

Human TNF alpha protein (active). GTX00102-pro

GTX00102-pro Image

SDS-PAGE analysis of GTX00102-pro Human TNF alpha protein (active).

Human TNF alpha protein (active). GTX00102-pro

GTX00102-pro Image

WB analysis of GTX00102-pro Human TNF alpha protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 259