APPLICATION
Application Note
TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-β are very similar to TNF-α, due to the similarity of molecular structure and the receptors. As reported, TNF-β could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-β (10 ng/ml) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope.
Observed MW
23 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.
Preservative
ProClin 300
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Pro36~Leu205 (NP_000586.2)
Expression System
E. coli
Purity
> 97%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
lymphotoxin alpha , LT , TNFB , TNFSF1 , TNLG1E
Background
The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]
Database
Research Area
DATA IMAGES
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GTX00103-pro Functional Assay Image
The apoptosis effect of GTX00103-pro Human TNF beta protein (active).
(A) A549 cells cultured in DMEM for 72hrs.
(B) A549 cells cultured in DMEM, stimulated with 10 ng/ml TNF-beta for 72hrs.
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GTX00103-pro Functional Assay Image
ELISA detection of IL-1 beta expression from A549 cells stimulated by GTX00103-pro Human TNF beta protein (active) at 10 ng/ml for 8hrs.
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GTX00103-pro Image
WB analysis of GTX00103-pro Human TNF beta protein (active).
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GTX00103-pro Image
SDS-PAGE analysis of GTX00103-pro Human TNF beta protein (active).
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REFERENCE
There are currently no references for Human TNF beta protein, His tag (active) (GTX00103-pro). Be the first to share your publications with this product.
REVIEW
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