APPLICATION
Application Note
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is less or equal to 1 ng/ml.
PROPERTIES
Form
Liquid
Buffer
20mM Tris-HCl buffer (pH8.0), 30% Glycerol, 100mM NaCl, 1mM DTT
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.25 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
N-terminal His-Tag; Length: 192 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL
Expression System
E. coli
Purity
> 90% by SDS-PAGE.
Endotoxin
< 1.0 EU/μg (determined by LAL method)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
lymphotoxin alpha , LT , TNFB , TNFSF1 , TNLG1E
Background
The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]
Database
Research Area
DATA IMAGES
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GTX67169-pro Image
3 μg of GTX67169-pro Human TNF beta protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain
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REFERENCE
There are currently no references for Human TNF beta protein, His tag (active) (GTX67169-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human TNF beta protein, His tag (active) (GTX67169-pro). Be the first to share your experience with this product.