GeneTex
  • Country / Location Selection

United States (US)

Human TNF beta protein (active)

Human TNF beta protein (active). GTX00103-pro
Human TNF beta protein (active). GTX00103-pro
Human TNF beta protein (active). GTX00103-pro
Human TNF beta protein (active). GTX00103-pro

Cat No. GTX00103-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-β are very similar to TNF-α, due to the similarity of molecular structure and the receptors. As reported, TNF-β could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-β (10 ng/ml) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope.

Observed MW

23 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro36~Leu205 (NP_000586.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

lymphotoxin alpha,LT,TNFB,TNFSF1,TNLG1E

Background

The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES
Human TNF beta protein (active). GTX00103-pro

GTX00103-pro Functional Assay Image

The apoptosis effect of GTX00103-pro Human TNF beta protein (active).
(A) A549 cells cultured in DMEM for 72hrs.
(B) A549 cells cultured in DMEM, stimulated with 10 ng/ml TNF-beta for 72hrs.

Human TNF beta protein (active). GTX00103-pro

GTX00103-pro Functional Assay Image

ELISA detection of IL-1 beta expression from A549 cells stimulated by GTX00103-pro Human TNF beta protein (active) at 10 ng/ml for 8hrs.

Human TNF beta protein (active). GTX00103-pro

GTX00103-pro Image

WB analysis of GTX00103-pro Human TNF beta protein (active).

Human TNF beta protein (active). GTX00103-pro

GTX00103-pro Image

SDS-PAGE analysis of GTX00103-pro Human TNF beta protein (active).

REFERENCE
There are currently no references for Human TNF beta protein (active) (GTX00103-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human TNF beta protein (active) (GTX00103-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 259