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Human TNFRSF14 protein, His tag

Human TNFRSF14 protein, His tag. GTX00145-pro
Human TNFRSF14 protein, His tag. GTX00145-pro
Human TNFRSF14 protein, His tag. GTX00145-pro

Cat. No. GTX00145-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($309)
See all TNFRSF14 protein products

APPLICATION

Application Note

TNFRSF14 (Tumor necrosis factor receptor superfamily member 14) belongs to the tumor necrosis factor receptor superfamily. TNFRSF14 functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. A binding ELISA assay was conducted to detect the association of TNFRSF14 with TNFa. Briefly, TNFRSF14 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl TNFRSF14 were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TNFRSF14 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF14 and TNFa was in a dose dependent manner.

Observed MW

22 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser41~Ser209 (NP_003811.2)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF receptor superfamily member 14 , ATAR , CD270 , HVEA , HVEM , LIGHTR , TR2

Background

This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Human TNFRSF14 protein, His tag. GTX00145-pro

GTX00145-pro Functional Assay Image

Functional ELISA analysis of GTX00145-pro Human TNFRSF14 protein which can bind immobilized TNF alpha protein.

Human TNFRSF14 protein, His tag. GTX00145-pro

GTX00145-pro Image

WB analysis of GTX00145-pro Human TNFRSF14 protein.

Human TNFRSF14 protein, His tag. GTX00145-pro

GTX00145-pro Image

SDS-PAGE analysis of GTX00145-pro Human TNFRSF14 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 309