GeneTex

  • Country / Location Selection

United States (US)

Human TWEAKR protein, His and GST tag

Human TWEAKR protein, His and GST tag. GTX00120-pro
Human TWEAKR protein, His and GST tag. GTX00120-pro
Human TWEAKR protein, His and GST tag. GTX00120-pro

Cat. No. GTX00120-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($309)
See all TWEAKR protein products

APPLICATION

Application Note

TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was in a dose dependent manner.

Observed MW

44 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Ser24~Ala126 (NP_057723.1)

Expression System

E. coli

Purity

> 80%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF receptor superfamily member 12A , CD266 , FN14 , TWEAKR

Database

Research Area

DATA IMAGES

Human TWEAKR protein, His and GST tag. GTX00120-pro

GTX00120-pro Functional Assay Image

Functional ELISA analysis of GTX00120-pro Human TWEAKR protein which can bind immobilized TNF alpha protein.

Human TWEAKR protein, His and GST tag. GTX00120-pro

GTX00120-pro Image

WB analysis of GTX00120-pro Human TWEAKR protein.

Human TWEAKR protein, His and GST tag. GTX00120-pro

GTX00120-pro Image

SDS-PAGE analysis of GTX00120-pro Human TWEAKR protein.

REFERENCE

There are currently no references for Human TWEAKR protein, His and GST tag (GTX00120-pro). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Human TWEAKR protein, His and GST tag (GTX00120-pro). Be the first to share your experience with this product.
Submit a Review
SDS
Proclin.pdf
Package List Price ($)
$ 309