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Human TWEAKR protein (active)

Human TWEAKR protein (active). GTX00120-pro
Human TWEAKR protein (active). GTX00120-pro
Human TWEAKR protein (active). GTX00120-pro

Cat No. GTX00120-pro

Application Functional Assay
Species Human
Package
10 μg ($289)
APPLICATION

Application Note

TNFRSF12A (Tumor necrosis factor receptor superfamily member 12A) belongs to the Tumor necrosis factor receptor superfamily. TNFRSF12A is thought to induce apoptosis weakly in some cell types and promotes angiogenesis and the proliferation of endothelial cells. A binding ELISA assay was conducted to detect the association of TNFRSF12A with TNFa. Briefly, TNFRSF12A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl TNFRSF12A were then transferred to TNFa-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TNFRSF12A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF12A and TNFa was in a dose dependent manner.

Observed MW

44 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Ser24~Ala126 (NP_057723.1)

Expression System

E. coli

Purity

> 80%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TNF receptor superfamily member 12A , CD266 , FN14 , TWEAKR

Database

Research Area

DATA IMAGES
Human TWEAKR protein (active). GTX00120-pro

GTX00120-pro Functional Assay Image

Functional ELISA analysis of GTX00120-pro Human TWEAKR protein (active) which can bind immobilized TNF alpha protein.

Human TWEAKR protein (active). GTX00120-pro

GTX00120-pro Image

SDS-PAGE analysis of GTX00120-pro Human TWEAKR protein (active).

Human TWEAKR protein (active). GTX00120-pro

GTX00120-pro Image

WB analysis of GTX00120-pro Human TWEAKR protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289