Human Tau (K18) protein, mutant P301L (Pre-formed Fibrils)

TEM image of GTX17675-pro Human Tau (K18) protein, mutant P301L (Pre-formed Fibrils).

TEM image of GTX17675-pro Human Tau (K18) protein, mutant P301L (Pre-formed Fibrils).

SDS-PAGE of ~15 kDa Human Tau (K18) protein, mutant P301L (preformed fibrils (PFFs)) (GTX17675-pro).
Lane 1: MW Ladder. Lane 2: Human Tau (K18) protein, mutant P301L (fibrils) (GTX17675-pro).

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau preformed fibrils (GTX17675-pro). Tau preformed fibrils (GTX17675-pro) seed the formation of new tau fibrils when combined with tau monomers (GTX17672-pro). Thioflavin T ex = 450 nm, em = 485 nm.