APPLICATION
Application Note
Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers.
PROPERTIES
Form
Liquid
Buffer
10 mM HEPES, 100 mM NaCl pH 7.4
Storage
Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Region/Sequence
Partial Tau without tagged; SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE
Expression System
E. coli
Purification
Purified by ion-exchange chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
microtubule associated protein tau , microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU
Database
Research Area
DATA IMAGES
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GTX17675-pro Image
SDS-PAGE of ~15 kDa Human Tau (K18) protein, mutant P301L (active, preformed fibrils (PFFs)) (GTX17675-pro).
Lane 1: MW Ladder. Lane 2: Human Tau (K18) protein, mutant P301L (active, fibrils) (GTX17675-pro).
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GTX17675-pro Image
TEM of Human Tau (K18) protein, mutant P301L (active, preformed fibrils (PFFs)) at 30kx magnification. HV=80kV. Fibrils were sonicated and stained with uranyl acetate.
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GTX17675-pro Image
TEM of Human Tau (K18) protein, mutant P301L (active, preformed fibrils (PFFs)) at 150kx magnification. HV=80kV. Fibrils were sonicated and stained with uranyl acetate.
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GTX17675-pro Image
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau preformed fibrils (GTX17675-pro). Tau preformed fibrils (GTX17675-pro) seed the formation of new tau fibrils when combined with tau monomers (GTX17672-pro). Thioflavin T ex = 450 nm, em = 485 nm.
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REFERENCE
There are currently no references for Human Tau (K18) protein, mutant P301L (active, Pre-formed Fibrils) (GTX17675-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human Tau (K18) protein, mutant P301L (active, Pre-formed Fibrils) (GTX17675-pro). Be the first to share your experience with this product.
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