GeneTex
  • Country / Location Selection

United States (US)

Human Thymidine Kinase 1 protein (active)

Human Thymidine Kinase 1 protein (active). GTX00185-pro
Human Thymidine Kinase 1 protein (active). GTX00185-pro
Human Thymidine Kinase 1 protein (active). GTX00185-pro

Cat No. GTX00185-pro

Application Functional Assay
Species Human
Package
10 μg ($299)
APPLICATION

Application Note

Thymidine kinase 1 (TK1), is a human thymidine kinase. Thymidine kinase has been making a growing impact in the cancer research community. It has been found that elevated blood serum levels of TK-1 correlates with metastatic capabilities of the cancer and thereby can be used to detect malignant types of cancer, furthermore TK-1 has been found to show up in blood serum even before clinical symptoms even start to show. Besides, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) has been identified as an interactor of TK1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TK1 and recombinant human GAPDH. Briefly, TK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to GAPDH-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-TK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of TK1 and GAPDH was in a dose dependent manner.

Observed MW

55 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; confidential (NP_003249.3)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

thymidine kinase 1,TK2

Background

The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES
Human Thymidine Kinase 1 protein (active). GTX00185-pro

GTX00185-pro Functional Assay Image

Functional ELISA analysis of GTX00185-pro Human Thymidine Kinase 1 protein (active) which can bind immobilized GAPDH protein.

Human Thymidine Kinase 1 protein (active). GTX00185-pro

GTX00185-pro Image

WB analysis of GTX00185-pro Human Thymidine Kinase 1 protein (active).

Human Thymidine Kinase 1 protein (active). GTX00185-pro

GTX00185-pro Image

SDS-PAGE analysis of GTX00185-pro Human Thymidine Kinase 1 protein (active).

REFERENCE
There are currently no references for Human Thymidine Kinase 1 protein (active) (GTX00185-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human Thymidine Kinase 1 protein (active) (GTX00185-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 299