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Human UCHL3 protein, His tag (active)

Human UCHL3 protein, His tag (active). GTX67178-pro

Cat. No. GTX67178-pro

Application

 Functional Assay

Species

 Human
Package
100 μg ($289)
See all UCHL3 protein products

APPLICATION

Application Note

Specific activity is > 1500 pmol/min/μg, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20mM Tris-HCl, 10% Glycerol, 1mM DTT

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 250 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA

Expression System

E. coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin C-terminal hydrolase L3 , UCH-L3

Background

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Human UCHL3 protein, His tag (active). GTX67178-pro

GTX67178-pro Image

3 μg of GTX67178-pro Human UCHL3 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 289