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Human UGCG protein, His tag

Human UGCG protein, His tag. GTX00280-pro
Human UGCG protein, His tag. GTX00280-pro
Human UGCG protein, His tag. GTX00280-pro

Cat. No. GTX00280-pro

Application

 Functional Assay

Species

 Human, Mouse, Rat
Package
10 μg ($309)
See all UGCG protein products

APPLICATION

Application Note

UDP Glucose Ceramide Glucosyltransferase (UGCG) is an enzyme which catalyzes the first glycosylation step in glycosphingolipid biosynthesis. It belongs to the glycosyltransferase 2 family. UGCG is widely expressed and transcription is upregulated during keratinocyte differentiation. Besides, Large Multifunctional Peptidase 2 (LMP2) has been identified as an interactor of UGCG, thus a binding ELISA assay was conducted to detect the interaction of recombinant human UGCG and recombinant human LMP2. Briefly, UGCG were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LMP2-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-UGCG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of UGCG and LMP2 was in a dose dependent manner.

Observed MW

16 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Lys39~Leu171 (NP_003349.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

UDP-glucose ceramide glucosyltransferase , GCS , GLCT1

Background

This gene encodes an enzyme that catalyzes the first glycosylation step in the biosynthesis of glycosphingolipids, which are membrane components containing lipid and sugar moieties. The product of this reaction is glucosylceramide, which is the core structure of many glycosphingolipids. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Human UGCG protein, His tag. GTX00280-pro

GTX00280-pro Functional Assay Image

Functional ELISA analysis of GTX00280-pro Human UGCG protein which can bind immobilized LMP2 protein.

Human UGCG protein, His tag. GTX00280-pro

GTX00280-pro Image

WB analysis of GTX00280-pro Human UGCG protein.

Human UGCG protein, His tag. GTX00280-pro

GTX00280-pro Image

SDS-PAGE analysis of GTX00280-pro Human UGCG protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 309