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Human VEGF Receptor 1 protein, His tag (active)

Human VEGF Receptor 1 protein, His tag (active). GTX01375-pro
Human VEGF Receptor 1 protein, His tag (active). GTX01375-pro
Human VEGF Receptor 1 protein, His tag (active). GTX01375-pro

Cat No. GTX01375-pro

Application

Functional Assay

Species

Human
Package
100 μg ($339),
50 μg ($259)

APPLICATION

Application Note

Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC cells. The ED₅₀ for this effect is typically 10-40 ng/ml in the presence of 10 ng/ml human VEGF165 protein.
2. Immobilized Human PLGF protein (active) (GTX01280-pro) at 10 μg/ml (100 μl/well) can bind human VEGFR1-his protein (GTX01375-pro). The EC₅₀ of human VEGFR1-his protein (GTX01375-pro) is 0.19-0.43 μg/ml.

Observed MW

35.6 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal poly His tagged; Met1-Ile328 of Human VEGF Receptor 1 protein (NP_002010.2)

Expression System

HEK293 cells

Purity

> 90% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

FLT,FLT-1,VEGFR-1,VEGFR1

Background

This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Human VEGF Receptor 1 protein, His tag (active). GTX01375-pro

GTX01375-pro Functional Assay Image

The protein activity of GTX01375-pro Human VEGF Receptor 1 protein was measured by its ability to inhibit VEGF-dependent induced HUVEC cells proliferation in the presence of 10 ng/ml recombinant human VEGF165.
ED₅₀ : 10-40 ng/ml

Human VEGF Receptor 1 protein, His tag (active). GTX01375-pro

GTX01375-pro Functional Assay Image

The protein binding activity was measured in a functional ELISA analysis of GTX01375-pro Human VEGF Receptor 1 protein which can bind immobilized GTX01280-pro Human PLGF protein.
Immobilized GTX01280-pro : 10 μg/ml (100 μl/well)
GTX01375-pro : 0.19-0.43 μg/ml

Human VEGF Receptor 1 protein, His tag (active). GTX01375-pro

GTX01375-pro Image

SDS-PAGE of 5 μg GTX01375-pro Human VEGF Receptor 1 protein, His tag (active).

REFERENCE

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REVIEW

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