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Human VEGF Receptor 2 protein (active)

Human VEGF Receptor 2 protein (active). GTX00259-pro
Human VEGF Receptor 2 protein (active). GTX00259-pro

Cat No. GTX00259-pro

Application

Functional Assay

Species

Human
Package
10 μg ($259)

APPLICATION

Application Note

Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) also known as kinase insert domain receptor acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. VEGFR2 functions as the primary mediator of vascular endothelial growth factor activation in endothelial cells. regulation of VEGFR-2 expression appears critical in mitogenesis, differentiation, and angiogenesis. To test the effect on inhibit the VEGF-dependent proliferation of endothelium cells, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM including 1μg/ml Vascular Endothelial Growth Factor C (VEGFC) and various concentrations of recombinant human VEGFR2. After incubated for 96h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of ECV-304 cells after incubation with VEGFR2 for 96h observed by inverted microscope.

Observed MW

90 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from PBS (pH 7.4) with 0.01% SKL, 1 mM DTT, 5% Trehalose, Proclin300. Reconstitute with 10 mM PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala20~Glu764 (NP_002244.1)

Expression System

293F cell

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

kinase insert domain receptor , CD309 , FLK1 , VEGFR , VEGFR2

Background

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Human VEGF Receptor 2 protein (active). GTX00259-pro

GTX00259-pro Functional Assay Image

The inhibition effect of GTX00259-pro Human VEGF Receptor 2 protein (active) on VEGF-dependent proliferation. Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated ECV-304 cells cultured in DMEM for 96hrs.
(B) ECV-304 cells cultured in DMEM, stimulated with 10 ng/ml VEGFR2 for 96hrs.

Human VEGF Receptor 2 protein (active). GTX00259-pro

GTX00259-pro Image

SDS-PAGE analysis of GTX00259-pro Human VEGF Receptor 2 protein (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 259