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Human VEGF121 protein (active)

Human VEGF121 protein (active). GTX00136-pro
Human VEGF121 protein (active). GTX00136-pro
Human VEGF121 protein (active). GTX00136-pro

Cat No. GTX00136-pro

Application Functional Assay
Species Human
Package
10 μg ($289)
APPLICATION

Application Note

VEGFA (Vascular endothelial growth factor A) is a growth factor and can be cleaved into several isoforms, including VEGF121. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It is accepted that the VEGF121 isoform stimulates the proliferation of vein endothelial cells. Thus, proliferation assay of recombinant human VEGF121 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard RPMI-1640  prior to the addition of various concentrations of VEGF121. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of ECV-304 cells after incubation with VEGF121 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant VEGF121 for 48h. And VEGF121 significantly increased cell viability of ECV-304 cells.

Observed MW

17/18 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro28~Arg147 (NP_001020537.2)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

vascular endothelial growth factor A,MVCD1,VEGF,VPF

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES
Human VEGF121 protein (active). GTX00136-pro

GTX00136-pro Functional Assay Image

Cell proliferation effect of GTX00136-pro Human VEGF121 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated ECV-304 cells cultured in RPMI-1640 for 48hrs.
(B) ECV-304 cells cultured in RPMI-1640, stimulated with 100 ng/ml VEGF121 for 48hrs.

Human VEGF121 protein (active). GTX00136-pro

GTX00136-pro Image

WB analysis of GTX00136-pro Human VEGF121 protein (active).

Human VEGF121 protein (active). GTX00136-pro

GTX00136-pro Image

SDS-PAGE analysis of GTX00136-pro Human VEGF121 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 289